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Sequence and phylogenetic analysis of the VP5 major outer capsid protein gene of H5N1 subtype of avian influenza virus.
Outbreak of avian influenza (AI) H5N1 viruses of subtype A in poultry and their infections to different mammalian species, including humans, are a severe threat to public health and the economy. The hemagglutinin (HA) subunit of the virion is one of the important surface proteins and considered a potential candidate for inclusion in a multivalent vaccine. To determine the relationship of AI H5N1 viruses isolated from humans, birds, and mammals, the sequence of the VP5 gene, another important surface protein on the influenza virus, was analyzed. Phylogenetic analysis revealed that the H5N1 viruses isolated from the birds and mammals formed two distinct lineages. One lineage includes those from China, Thailand, and Vietnam and the other includes viruses from Indonesia, Malaysia, and Egypt. The two lineages are separated by a common ancestor and also infected humans. Sequence analysis showed that the VP5 gene of the viruses isolated from humans in 2003 and 2004 in Hong Kong are closely related. Further analysis revealed that these viruses have evolved from a previously circulating avian virus in 1997 in Hong Kong and recent outbreaks in southern China. This study confirmed that there was a new H5N1 subtype circulating in Hong Kong that was also responsible for the recent outbreak in humans in 2004.A new scalable, robotically-assisted technique for minimally invasive heart valve surgery.
We have developed a new, minimally invasive robot-assisted technique (MIRATS) for aortic valve surgery that employs a new robotic instrumentation system (DISCOVER-F). A fully robotic, multi-jointed, catheter-guided robot (AUTOPILOT) was used to assist with aortic cann