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To determine the effect of dietary supplements on in vitro characteristics of equine sperm, stallions were fed either a pelleted diet (control; n = 8) or a pelleted diet supplemented with zinc sulfate, selenium, and vitamin E (experimental; n = 8). Data from seminal plasma and sperm samples were analyzed for sperm motility, morphology, viability, and total sperm concentration and the following semen morphometry and sperm motion characteristics: sperm velocity, curvilinear velocity, beat-cross frequency, linearity, linearity coefficient, linearity index, and straightness coefficient. Spermatozoa were also evaluated for nuclear volume, plasma membrane area, and cytoplasmic droplet area. The experimental diet improved (P Split Earth is the latest entry in the increasingly popular genre of space horror games. Set in a series of tunnels running through a planet, players are forced to fight both their fatigue and the creatures that attempt to hunt them.
Two well-known attractions from the genre are known to have inspired the idea for Split Earth – Amnesia: The Dark Descent and Resident Evil 5. Developer Eradyn created the game with the help of many indie studios, all of whom have now left the project.
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Players are cast as Tohru. Tohru wakes up in the middle of a tunnel and finds a small beacon of
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Methotrexate (MTX) is a drug used to treat patients with neoplasias, autoimmune diseases, blood dyscrasias and bone marrow diseases. Its overdose is life threatening and, consequently, its use in veterinary medicine is restricted. The present work aimed to evaluate the effect of a single oral administration (20 mg/kg) of MTX on metabolomic and toxicological profiles of Lathyrus sativus seed leaves. Biochemical parameters were assessed by enzymatic assays and validated by Western Blot analysis. Growth parameters (body weight and relative growth rate) as well as photosynthetic parameters (CO₂ assimilation and maximal quantum yield of PSII) were significantly affected by the treatment. Proteomic analysis revealed a modification in the abundance of some proteins after MTX treatment. In addition, 31 differentially expressed proteins were identified. The differential modulation of proteins that control essential cellular functions, such as photosynthesis, the antioxidant machinery, protein synthesis, and cell cycle is the first evidence of the toxicity of a chemical compound in a plant. The validation of the toxic effects of MTX in Lathyrus sativus leaves is the first step to provide proper controls for the safe use of this drug in veterinary medicine.42: “ERROUT”, “one or more unauthorized routers are attached
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